RAPID AND SENSITIVE TYPING OF FORENSIC STAINS BY PCR AMPLIFICATION OF POLYMORPHIC SIMPLE REPEAT SEQUENCES IN CASE WORK

被引：63

作者：

ROEWER, L ^{[1
]}

EPPLEN, JT ^{[1
]}

机构：

[1] MAX PLANCK INST PSYCHIAT,W-8033 MARTINSRIED,GERMANY

来源：

FORENSIC SCIENCE INTERNATIONAL | 1992年 / 53卷 / 02期

关键词：

IDENTIFICATION TECHNIQUES; HYPERVARIABLE SIMPLE REPEAT LOCI; CONTAMINATION PROBLEM;

D O I：

10.1016/0379-0738(92)90193-Z

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

After failure of conventional typing and multilocus DNA fingerprinting methods to compare a minute vaginal swab stain with blood from a murder victim and a suspect, we used enzymatic DNA amplification (polymerase chain reaction, PCR) to discriminate the DNAs by typing of simple sequence lengths polymorphisms. A mixed dinucleotide locus in the HLA-DRB gene region and three novel tetranucleotide polymorphisms located autosomally as well as on the human Y chromosome were used to exclude a jailed subject from a case of murder.

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页码：163 / 171

页数：9

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