DIFFERENTIAL UP-REGULATION BY TRANSFER-RNAS OF RIBOSOME-INACTIVATING PROTEINS

被引：19

作者：

BRIGOTTI, M ^{[1
]}

CARNICELLI, D ^{[1
]}

ALVERGNA, P ^{[1
]}

PALLANCA, A ^{[1
]}

SPERTI, S ^{[1
]}

MONTANARO, L ^{[1
]}

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO PATOL SPERIMENTALE,I-40126 BOLOGNA,ITALY

来源：

FEBS LETTERS | 1995年 / 373卷 / 02期

关键词：

RIBOSOME-INACTIVATING PROTEIN; GELONIN; AGROSTIN; PARLEY RIP; PAP; TRITIN; LUFFIN; TRICHOKIRIN; TRICHOSANTHIN; TRANSFER-RNA;

D O I：

10.1016/0014-5793(95)01023-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Some plant ribosome-inactivating proteins (RIPs) with RNA-N-glycosidase activity on 28S RNA require, for the inactivation of ribosomes, the presence of macromolecular cofactors present in post-ribosomal supernatants. In the case of gelonin one of the cofactors is tRNA(Trp) lacking one or two nucleotides at the 3'-CCA end [Brigotti, M., Carnicelli, D., Alvergna, P., Pallanca, A., Lorenzetti, R., Denaro, M., Sperti, S. and Montanaro, L. (1995) Biochem. J. 310, 249-253]. In the present study it is shown that tRNAs are involved in the up-regulation of all the cofactor-requiring RIPs up to now identified (agrostin, barley RIP, PAP and tritin, besides gelonin). Polyacrylamide gel electrophoresis shows that tRNA fractions with different mobilities stimulate different RIPs. With the identification of agrostin, the cofactor-requiring RIPs (italics) add to five out of a total of thirteen investigated: barley RIP, bryodin-R, gelonin, lychnin, momordin, momorcochin-S, PAP, saporin-6, tritin [Carnicelli, D., Brigotti, M., Montanaro, L. and Sperti, S. (1992) Biochem. Biophys. Res. Commun. 182, 579-582], agrostin, luffin, trichokirin and trichosanthin (present study).

引用

页码：115 / 118

页数：4

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