ONLINE LIQUID-CHROMATOGRAPHY FAST-ATOM BOMBARDMENT OF PROTEIN TRYPTIC DIGESTS

被引：14

作者：

BELL, DJ

BRIGHTWELL, MD

NEVILLE, WA

WEST, A

机构：

[1] Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Surrey, KT18 5XQ, Great Burgh, Yew Tree Bottom Road, Epsom

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 03期

关键词：

D O I：

10.1002/rcm.1290040306

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The assignment of the peptides in the chromatographic separation of tryptic peptides from a large protein was investigated by liquid chromatography/fast‐atom bombardment mass spectrometry (LC/FABMS). Methodical interfacing of the instrumentation was required to maintain the resolution of the 70 peptides in the mixture whilst gaining maximum fast‐atom bombardment sensitivity. The reconstructed ion current showed excellent correlation with ultra violet detection, illustrating that chromatographic resolution was indeed preserved. Results allowed the determination of retention times of peptides by plotting the mass chromatograms of the expected materials from the acquired spectra. It was also found that spectrum‐averaging and background subtraction could yield considerable sequence information from the LC/FAB spectra of peptides, acquired using a two‐sector instrument. Copyright © 1990 Heyden & Son Limited

引用

页码：88 / 91

页数：4

共 10 条

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