A SIMPLE NONISOTOPIC METHOD FOR THE QUANTITATION OF RED CELL-BOUND IMMUNOGLOBULIN

被引：54

作者：

KUMPEL, BM

机构：

[1] UK Transplant Service, Bristol

来源：

VOX SANGUINIS | 1990年 / 59卷 / 01期

关键词：

D O I：

10.1111/j.1423-0410.1990.tb02111.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Abstract. A method was developed for the estimation of the number of molecules of immunoglobulin bound to red cells. Solubilization of sensitized, washed red cells was followed by an enzyme‐linked immunosorbent assay of immunoglobulin concentration (SOL‐ELISA). This assay gave results that were comparable to isotopic methods, was reproducible, utilised inexpensive reagents and was relatively quick and simple to perform. © 1990 S. Karger AG, Basel

引用

收藏

页码：34 / 38

页数：5

相关论文

共 17 条

[1]

AVENT ND, 1988, THESIS U BRISTOL, P159

[2] STUDIES WITH MARKED ANTISERA - QUANTITATIVE STUDIES WITH ANTISERA MARKED WITH IODINE ISOTOPE-131 AND THEIR CORRESPONDING RED-CELL ANTIGENS [J].

BOURSNELL, JC ;

COOMBS, RRA ;

RIZK, V .

BIOCHEMICAL JOURNAL, 1953, 55 (05) :745-758

[3] BLOCKADE OF CLEARANCE OF IMMUNE-COMPLEXES BY AN ANTI-FC-GAMMA-RECEPTOR MONOCLONAL-ANTIBODY [J].

CLARKSON, SB ;

KIMBERLY, RP ;

VALINSKY, JE ;

WITMER, MD ;

BUSSEL, JB ;

NACHMAN, RL ;

UNKELESS, JC .

JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (02) :474-489

[4] 3 EPITOPES ON THE HUMAN RH ANTIGEN-D RECOGNIZED BY I-125-LABELED HUMAN MONOCLONAL IGG ANTIBODIES [J].

GORICK, BD ;

THOMPSON, KM ;

MELAMED, MD ;

HUGHESJONES, NC .

VOX SANGUINIS, 1988, 55 (03) :165-170

[5] STUDIES ON REACTION BETWEEN BLOOD-GROUP ANTIBODY ANTI-D AND ERYTROCYTES [J].

HUGHESJONES, NC ;

GARDNER, B ;

TELFORD, R .

BIOCHEMICAL JOURNAL, 1963, 88 (03) :435-&

[6] RADIOIMMUNOASSAY OF THE FUNCTIONAL-ACTIVITY OF ANTI-D (RH) PREPARATIONS USING A HUMAN MONOCLONAL I-125 LABELED ANTI-D [J].

HUGHESJONES, NC ;

DAWES, B ;

GORICK, BD ;

MELAMED, MD ;

PEPPER, R ;

THOMPSON, KM .

VOX SANGUINIS, 1987, 53 (03) :175-180

[7] QUANTITATION OF RED CELL-BOUND IMMUNOGLOBULIN AND COMPLEMENT USING ENZYME-LINKED ANTIGLOBULIN CONSUMPTION ASSAY [J].

KIRUBA, R ;

HAN, P .

TRANSFUSION, 1988, 28 (06) :519-524

[8] HUMAN MONOCLONAL ANTI-D ANTIBODIES .1. THEIR PRODUCTION, SEROLOGY, QUANTITATION AND POTENTIAL USE AS BLOOD GROUPING REAGENTS [J].

KUMPEL, BM ;

POOLE, GD ;

BRADLEY, BA .

BRITISH JOURNAL OF HAEMATOLOGY, 1989, 71 (01) :125-129

[9]

LAMBERT R, 1978, MED LAB SCI, V35, P233

[10] HUMAN MONOCLONAL ANTI-D WITH REACTIVITY AGAINST CATEGORY DVI CELLS USED IN BLOOD GROUPING AND DETERMINATION OF THE INCIDENCE OF THE CATEGORY DVI PHENOTYPE IN THE DU POPULATION [J].

LEADER, KA ;

KUMPEL, BM ;

POOLE, GD ;

KIRKWOOD, JT ;

MERRY, AH ;

BRADLEY, BA .

VOX SANGUINIS, 1990, 58 (02) :106-111