ADP-RIBOSYLATION OF NUCLEOLAR PROTEINS IN HELA TUMOR-CELLS

被引：51

作者：

LEITINGER, N ^{[1
]}

WESIERSKAGADEK, J ^{[1
]}

机构：

[1] UNIV VIENNA,INST TUMORBIOL CANC RES,BORSCHKEGASSE 8A,A-1090 VIENNA,AUSTRIA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1993年 / 52卷 / 02期

关键词：

POLYACRYLAMIDE GEL ELECTROPHORESIS; RIBOSOMAL GENES; POSTTRANSLATIONAL MODIFICATION;

D O I：

10.1002/jcb.240520207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with P-32-NAD; then the nucleolar proteins were analyzed by 1 -dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation.

引用

页码：153 / 158

页数：6

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