MOLECULAR-CLONING AND CHARACTERIZATION OF CATECHOL 2,3-DIOXYGENASES FROM BIPHENYL POLYCHLORINATED BIPHENYLS-DEGRADING BACTERIA

被引：7

作者：

CHANG, H

LEE, J

ROH, S

KIM, SR

MIN, KR

KIM, CK

KIM, EG

KIM, Y

机构：

[1] CHUNGBUK NATL UNIV,COLL PHARM,DEPT BIOCHEM,CHONGJU 360763,SOUTH KOREA

[2] CHUNGBUK NATL UNIV,COLL MED,CHONGJU 360763,SOUTH KOREA

[3] CHUNGBUK NATL UNIV,COLL NAT SCI,CHONGJU 360763,SOUTH KOREA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91238-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Catechol 2,3-dioxygenases were cloned from Alcaligenes sp. KF711, Pseudomonas putida KF715, and Achromobacter xylosoxidans KF701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. All of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (PAGE). The purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. The catechol 2,3-dioxygenase from Alcaligenes sp. KF711 exhibited higher ring-fission activity to 4-chlorocatechol than those from P. putida KF715 and A. xylosoxidans KF701. In electrophoretic mobilities, the three enzymes were different from one another on nondenaturing PAGE but the same on SDS-PAGE. © 1992.

引用

页码：609 / 614

页数：6

共 20 条

[1] CLONING AND EXPRESSION OF GENES INVOLVED IN 4-CHLOROBIPHENYL TRANSFORMATION BY PSEUDOMONAS-TESTOSTERONI - HOMOLOGY TO POLYCHLOROBIPHENYL-DEGRADING GENES IN OTHER BACTERIA [J].