ATRIAL STRETCH INDUCES RAPID INCREASE IN BRAIN NATRIURETIC PEPTIDE BUT NOT IN ATRIAL-NATRIURETIC-PEPTIDE GENE-EXPRESSION IN-VITRO

被引:105
作者
MANTYMAA, P [1 ]
VUOLTEENAHO, O [1 ]
MARTTILA, M [1 ]
RUSKOAHO, H [1 ]
机构
[1] UNIV OULU, DEPT PHYSIOL, SF-90220 OULU, FINLAND
关键词
D O I
10.1210/en.133.3.1470
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Pressure and volume overload in vivo is characterized by induction of the expression of two cardiac hormones, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), but whether stretch directly or other pathophysiological factors associated with cardiac overload cause the activation of these genes is not known. In the present study we examined the effect of short-term (from 30 min to 2 h) direct myocardial stretch on atrial ANP and BNP synthesis and release in modified perfused rat heart preparation that enabled the stepwise distension of the right atrium by pressures approximating those found in vivo. The increase in right atrial pressure by 3.6 mm Hg for 2 h resulted in a 3.3- (p<0.00 1) and 1. 7-fold (p<0.02) increase in the rate of IR-ANP and IR-BNP release, respectively, into the perfusate. The maximal increase in both ANP and BNP release was seen after 20 min distension. Thereafter the perfusate IR-ANP and IR-BNP concentration gradually decreased, reaching control values within 2 hours. Chromatographic analysis showed that the hearts primarily release the active, processed 28- and 45-amino acid ANP- and BNP-like peptides, respectively, both before and during atrial stretch. Atrial stretch induced rapid stimulation of BNP gene expression: 1.9- (p<0.001) and 4.5-fold (p<0.001) increase in right auricular BNP mRNA levels after 1.0 and 2. 0 hours' stretching, respectively, was found on Northern blot analysis, while no change was seen after 30 min distension. In contrast, stretching for up to 2 h did not change auricular ANP mRNA, IR-ANP or IR-BNP levels. Our results show for the first time that atrial stretch induces rapid stimulation of both synthesis and secretion of BNP. The induction of BNP gene expression in the very early stages of cardiac overload mimics the induction of protooncogenes and occurred without involvement of humoral or neural factors. The lack of response of atrial ANP mRNA levels demonstrates that the regulation of BNP gene expression differs from that of ANP.
引用
收藏
页码:1470 / 1473
页数:4
相关论文
共 31 条
  • [1] AKASHI M, 1991, BLOOD, V78, P2005
  • [2] REGULATION OF CARDIAC GENE-EXPRESSION DURING MYOCARDIAL GROWTH AND HYPERTROPHY - MOLECULAR STUDIES OF AN ADAPTIVE PHYSIOLOGICAL-RESPONSE
    CHIEN, KR
    KNOWLTON, KU
    ZHU, H
    CHIEN, S
    [J]. FASEB JOURNAL, 1991, 5 (15) : 3037 - 3046
  • [3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
    CHIRGWIN, JM
    PRZYBYLA, AE
    MACDONALD, RJ
    RUTTER, WJ
    [J]. BIOCHEMISTRY, 1979, 18 (24) : 5294 - 5299
  • [4] INCREASED TRANSCRIPTS FOR B-TYPE NATRIURETIC PEPTIDE IN SPONTANEOUSLY HYPERTENSIVE RATS - QUANTITATIVE POLYMERASE CHAIN-REACTION FOR ATRIAL AND BRAIN NATRIURETIC PEPTIDE TRANSCRIPTS
    DAGNINO, L
    LAVIGNE, JP
    NEMER, M
    [J]. HYPERTENSION, 1992, 20 (05) : 690 - 700
  • [5] ALIGNMENT OF RAT CARDIONATRIN SEQUENCES WITH THE PREPROCARDIONATRIN SEQUENCE FROM COMPLEMENTARY-DNA
    FLYNN, TG
    DAVIES, PL
    KENNEDY, BP
    DEBOLD, ML
    DEBOLD, AJ
    [J]. SCIENCE, 1985, 228 (4697) : 323 - 325
  • [6] VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY
    FORT, P
    MARTY, L
    PIECHACZYK, M
    ELSABROUTY, S
    DANI, C
    JEANTEUR, P
    BLANCHARD, JM
    [J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (05) : 1431 - 1442
  • [7] GENE FOR THE RAT ATRIAL NATRIURETIC PEPTIDE IS REGULATED BY GLUCOCORTICOIDS INVITRO
    GARDNER, DG
    GERTZ, BJ
    DESCHEPPER, CF
    KIM, DY
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (04) : 1275 - 1281
  • [8] STIMULATION OF 3T3 CELLS INDUCES TRANSCRIPTION OF THE C-FOS PROTO-ONCOGENE
    GREENBERG, ME
    ZIFF, EB
    [J]. NATURE, 1984, 311 (5985) : 433 - 438
  • [9] LIGHT AND ELECTRON-MICROSCOPIC LOCALIZATION OF BRAIN NATRIURETIC PEPTIDE IN RELATION TO ATRIAL-NATRIURETIC-PEPTIDE IN PORCINE ATRIUM - IMMUNOHISTOCYTOCHEMICAL STUDY USING SPECIFIC MONOCLONAL-ANTIBODIES
    HASEGAWA, K
    FUJIWARA, H
    ITOH, H
    NAKAO, K
    FUJIWARA, T
    IMURA, H
    KAWAI, C
    [J]. CIRCULATION, 1991, 84 (03) : 1203 - 1209
  • [10] OCCURRENCE OF A NOVEL CARDIAC NATRIURETIC PEPTIDE IN RATS
    ITOH, H
    NAKAO, K
    KAMBAYASHI, Y
    HOSODA, K
    SAITO, Y
    YAMADA, T
    MUKOYAMA, M
    ARAI, H
    SHIRAKAMI, G
    SUGA, S
    YOSHIDA, I
    INOUYE, K
    IMURA, H
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) : 732 - 739