SERUM-FREE CULTIVATION OF PLASMODIUM-FALCIPARUM GAMETOCYTES IN-VITRO

被引：13

作者：

LINGNAU, A ^{[1
]}

MARGOS, G ^{[1
]}

MAIER, WA ^{[1
]}

SEITZ, HM ^{[1
]}

机构：

[1] UNIV BONN, INST MED PARASITOL, SIGMUND FREUD STR 25, W-5300 BONN 1, GERMANY

来源：

PARASITOLOGY RESEARCH | 1993年 / 79卷 / 05期

关键词：

D O I：

10.1007/BF00931826

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Several components were tested for their ability to replace human serum in cultures of Plasmodium falciparum set up for the development of gametocytes. Besides a serum-free medium, A*I*M*V (Gibco BRL), RPMI medium supplemented with commercially available serum substitutes was used to culture gametocytes. The following substances served as serum replacements: Basal Medium Supplement (Biochrom), Ultroser G (Gibco BRL) and Nutridoma-SR (Boehringer Mannheim). All serum-free additives supported some parasite growth, but only in RPMI supplemented with Nutridoma-SR were morphologically mature gametocytes obtained. The asexual forms developed almost as well as in RPMI with human serum added.

引用

页码：378 / 384

页数：7

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