APPLICATION OF CAPILLARY DNA CHROMATOGRAPHY TO DETECT AIDS VIRUS (HIV-1) DNA IN BLOOD

被引:12
作者
BROWNLEE, RG
SUNZERI, FJ
BUSCH, MP
机构
[1] Irwin Memorial Blood Bank, San Francisco
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 533卷
关键词
D O I
10.1016/S0378-4347(00)82189-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The application of capillary DNA chromatography to the detection of the AIDS human immunodeficiency virus, type 1 (HIV-1) proviral DNA in blood is reported for the first time. Combining polymerase chain reaction with fluorescence-labeled DNA probes provides the basis for the amplification and specific detection of the DNA. Samples analyzed for HIV-1 DNA included both infected human blood and cell cultures. The new DNA separation method, capillary DNA chromatography (related to both capillary electrophoresis and capillary hydrodynamic chromatography), is shown to be a powerful method of analysis for DNA. These preliminary results indicate that an automated approach to screening the blood supply for HIV-1 may become a reality in the future. © 1990.
引用
收藏
页码:87 / 96
页数:10
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