MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY OF VITAMIN B-6 WITH ELECTROCHEMICAL DETECTION

被引：72

作者：

YIK, YF ^{[1
]}

LEE, HK ^{[1
]}

LI, SFY ^{[1
]}

KHOO, SB ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,DEPT CHEM,SINGAPORE 0511,SINGAPORE

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 585卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)85067-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A system for interfacing electrochemical detection with micellar electrokinetic capillary chromatography is demonstrated. This system couples the separation column to a short length of the same column material together with a section of porous graphite tubing which forms an electrically conductive joint. The joint is immersed in a buffer reservoir together with the ground electrode of the high-power source (15 kV). The reservoir is electrically insulated from the electrochemical cell containing the carbon fibre detector. This configuration effectively separates the detector from the high separation potential applied. Amperometric detection with micellar solutions is demonstrated for a mixture of B-6 vitamers on a 50-mu-m I.D. column. A detection limit of ca. 4 fmol is obtained. The linear dynamic range of the calibration plot is slightly over two orders of magnitude (from ca. 1 to 200 ppm).

引用

页码：139 / 144

页数：6

共 15 条

[1] THE USE OF PLATINUM MICROELECTRODES FOR ELECTROCHEMICAL INVESTIGATIONS IN LOW-TEMPERATURE GLASSES OF NON-AQUEOUS SOLVENTS [J].