ROLE OF MEMBRANE PHOSPHOLIPIDS AND GLYCOLIPIDS IN CELL-TO-CELL FUSION BY VSV

被引:7
作者
CONTI, C
MASTROMARINO, P
ORSI, N
机构
[1] Istituto di Microbiologia, Facoltà di Medicina, Università di Roma La Sapienza, 00100 Roma
关键词
VESICULAR STOMATITIS VIRUS; SYNCYTIUM; FUSION FROM WITHOUT; LIPID; RECEPTOR;
D O I
10.1016/0147-9571(91)90136-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To identify membrane components of CER cells interacting with vesicular stomatitis virus (VSV) during fusion at acidic pH (fusion from without, FFWO) two different approaches have been used, i.e. (i) treating the whole cells with enzymes and (ii) testing the ability of isolated membrane molecules to interfere with FFWO. Phospholipase A2 and C digestion of cells greatly reduced syncytia formation, pointing towards the involvement of lipid structures as target sites for VSV. Cell susceptibility to FFWO was also reduced after neuraminidase, beta-galactosidase or periodate treatment, suggesting that carbohydrate residues may participate in a complex receptor structure required for virus fusion. When membrane molecules were examined separately for their ability to inhibit viral FFWO, phosphatidylserine, phosphatidylinositol, sphingomyelin, cholesterol and GM3 ganglioside were found to be active, confirming the role of membrane lipid moiety in the cell surface structures involved in the early phases of VSV infection.
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页码:303 / &
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