HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETECTION OF ENANTIOMERIC AMINO-ALCOHOLS AFTER DERIVATIZATION WITH ORTHO-PHTHALDIALDEHYDE AND VARIOUS THIOSUGARS

被引：11

作者：

JEGOROV, A

TRNKA, T

STUCHLIK, J

机构：

[1] CHARLES UNIV, DEPT ORGAN CHEM, CS-12840 PRAGUE, CZECHOSLOVAKIA

[2] GALENA CO, CS-14770 OPAVA, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)80138-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A series of thiosugars are described as the new pre-column derivatization agents for chiral resolution of 2-amino-l-alcohols. The isoindole derivatives were formed by reaction of 2-amino-l-alcohols with o-phthaldialdehyde and thiosugars in aqueous solution and at a mildly basic pH. The reaction was complete within 1 min. The diastereomers formed can efficiently be resolved on conventional reversed-phase columns and measured with a fluorescence detector which provides detection limits of less than 10 pmol per 10-mu-l injection.

引用

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页码：311 / 317

页数：7

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