BIODIAGNOSTICS AND POLYMER IDENTIFICATION WITH MULTIPLEX DYES

被引：25

作者：

SEEGER, S ^{[1
]}

BACHTELER, G ^{[1
]}

DREXHAGE, KH ^{[1
]}

ARDENJACOB, J ^{[1
]}

DELTAU, G ^{[1
]}

GALLA, K ^{[1
]}

HAN, KT ^{[1
]}

MULLER, R ^{[1
]}

KOLLNER, M ^{[1
]}

RUMPHORST, A ^{[1
]}

SAUER, M ^{[1
]}

SCHULZ, A ^{[1
]}

WOLFRUM, J ^{[1
]}

机构：

[1] UNIV GESAMTHSCH SIEGEN,INST PHYS CHEM,D-57068 SIEGEN,GERMANY

来源：

BERICHTE DER BUNSEN-GESELLSCHAFT-PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 1993年 / 97卷 / 12期

关键词：

BIOPHYSICAL CHEMISTRY; FLUORESCENCE; IMMUNOASSAY; POLYMERS; TIME-RESOLVED FLUORESCENCE SPECTROSCOPY;

D O I：

10.1002/bbpc.19930971208

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A new analytical concept based on time-resolved fluorescence spectroscopy is presented, which uses the characteristic fluorescence lifetimes as information to identify new fluorescent dyes, called multiplex dyes. Several dyes can be distinguished at nearly fixed excitation and emission wavelength by recognition of the lifetimes. A pattern recognition technique is used for data analysis. As light sources a pulsed laser diode is used. First experiments are shown in several application fields, like DNA sequencing, immunoassays and tagging of polymer material.

引用

页码：1542 / 1548

页数：7

共 14 条

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