PROMOTER SPECIFICITY AND DELETION ANALYSIS OF 3 HEAT-STRESS TRANSCRIPTION FACTORS OF TOMATO

被引：105

作者：

TREUTER, E ^{[1
]}

NOVER, L ^{[1
]}

OHME, K ^{[1
]}

SCHARF, KD ^{[1
]}

机构：

[1] INST PLANT BIOCHEM,06120 HALLE,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1993年 / 240卷 / 01期

关键词：

TOMATO; HEAT STRESS; TRANSCRIPTION FACTOR; TRANSIENT EXPRESSION ASSAY;

D O I：

10.1007/BF00276890

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transient expression assays in transformed tobacco (Nicotiana plumbaginifolia) mesophyll protoplasts were used to test the activity of three tomato heat stress transcription factors, HSF24, HSF8 and HSF30, in a trans-activation and a trans-repression assay. The results document differences between the three HSFs with respect to their response to the configuration of heat stress promoter elements (HSEs) in the reporter construct (promoter specificity) and to the stress regime used for activation. Analysis of C-terminal deletions identified acidic sequence elements with a central tryptophan residue, which are important for HSF activity control. Surprisingly, heterologous HSFs from Drosophila and human cells, but not from yeast, were also functional as heat stress-induced transcription factors in this tobacco protoplast system.

引用

页码：113 / 125

页数：13

共 51 条

[1] ATTENUATION OF THE HEAT-SHOCK RESPONSE IN HELA-CELLS IS MEDIATED BY THE RELEASE OF BOUND HEAT-SHOCK TRANSCRIPTION FACTOR AND IS MODULATED BY CHANGES IN GROWTH AND IN HEAT-SHOCK TEMPERATURES [J].