CONSTRUCTION AND PRELIMINARY-ANALYSIS OF THE ICRF HUMAN P1 LIBRARY

被引：32

作者：

FRANCIS, F

ZEHETNER, G

HOGLUND, M

LEHRACH, H

机构：

[1] Genome Analysis Laboratory, Imperial Cancer Research Fund (ICRF), London, England

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1994年 / 11卷 / 5-6期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/1050-3862(94)90035-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

P1 clone libraries have now been established as effective complements to cosmid and yeast artificial chromosome libraries in long-range mapping projects. To allow, general access to P1 clones, we have constructed human and mouse P1 libraries. Clones have been picked into microtiter plates and used to prepare high-density filter grids, providing an efficient and easy screening system. Filters are being made available to other laboratories through the Reference Library System. In this work, we have developed a reliable protocol for generating P1 clones, based on the use of pulsed-field gel electrophoresis for size selection of DNA. A 1.2x genome coverage human library has been produced using this method. A preliminary analysis of this library is described.

引用

页码：148 / 157

页数：10

共 32 条

[1] ALBERTSEN HM, 1990, P NATL ACAD SCI USA, V87, P5109
[2] CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOME LIBRARIES WITH LARGE INSERTS USING FRACTIONATION BY PULSED-FIELD GEL-ELECTROPHORESIS
ANAND, R
VILLASANTE, A
TYLERSMITH, C
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (09) : 3425 - 3433
[3] A COSMID CONTIG AND HIGH-RESOLUTION RESTRICTION MAP OF THE 2 MEGABASE REGION CONTAINING THE HUNTINGTONS-DISEASE GENE
BAXENDALE, S
MACDONALD, ME
MOTT, R
FRANCIS, F
LIN, C
KIRBY, SF
JAMES, M
ZEHETNER, G
HUMMERICH, H
VALDES, J
COLLINS, FS
DEAVEN, LJ
GUSELLA, JF
LEHRACH, H
BATES, GP
[J]. NATURE GENETICS, 1993, 4 (02) : 181 - &
[4] THE DIRECT SCREENING OF COSMID LIBRARIES WITH YAC CLONES
BAXENDALE, S
BATES, GP
MACDONALD, ME
GUSELLA, JF
LEHRACH, H
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (23) : 6651 - 6651
[5] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS
BURKE, DT
CARLE, GF
OLSON, MV
[J]. SCIENCE, 1987, 236 (4803) : 806 - 812
[6] GENOMIC SEQUENCING
CHURCH, GM
GILBERT, W
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 1991 - 1995
[7] GENOME LINKING WITH YEAST ARTIFICIAL CHROMOSOMES
COULSON, A
WATERSTON, R
KIFF, J
SULSTON, J
KOHARA, Y
[J]. NATURE, 1988, 335 (6186) : 184 - 186
[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[9] MAPPING THE DROSOPHILA GENOME WITH YEAST ARTIFICIAL CHROMOSOMES
GARZA, D
AJIOKA, JW
BURKE, DT
HARTL, DL
[J]. SCIENCE, 1989, 246 (4930) : 641 - 646
[10] ISOLATION OF A PUTATIVE TRANSCRIPTIONAL REGULATOR FROM THE REGION OF 22Q11 DELETED IN DIGEORGE-SYNDROME, SHPRINTZEN SYNDROME AND FAMILIAL CONGENITAL HEART-DISEASE
HALFORD, S
WADEY, R
ROBERTS, C
DAW, SCM
WHITING, JA
ODONNELL, H
DUNHAM, I
BENTLEY, D
LINDSAY, E
BALDINI, A
FRANCIS, F
LEHRACH, H
WILLIAMSON, R
WILSON, DI
GOODSHIP, J
CROSS, I
BURNS, J
SCAMBLER, PJ
[J]. HUMAN MOLECULAR GENETICS, 1993, 2 (12) : 2099 - 2107

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