IDENTIFICATION OF MYO-INOSOSE-2 AND SCYLLO-INOSITOL IN MAMMALIAN TISSUES

被引:33
作者
SHERMAN, WR
STEWART, MA
SIMPSON, PC
GOODWIN, SL
机构
[1] Department of Psychiatry, Washington University School of Medicine, St. Louis, Missouri
关键词
D O I
10.1021/bi00842a040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
myo-Inosose-2 has been identified as a micromolar constituent of rat sciatic nerve and calf brain by isolation of its trimethylsilyl (TMS) ether using gas chromatography. TMS-mvo-inosose-2 was obtained from nerve in nanogram amounts and was identified by two means: (1) hydrolysis to free myo-inosose- 2, conversion into the oxime, resilylation, and gas chromatographic comparison with authentic TMS-myo-inosose-2 oxime; (2) hydrolysis, reduction with sodium amalgam, silylation, and chromatographic identification of the product as TMS-myo-inositol. By serial gas chromatography on two types of columns TMS-myo-inosose-2 was obtained in microgram amounts from calf brain. The inosose was found to be identical with authentic TMS-myo-inosose-2 by combined gas chromatography-mass spectrometry. scyllo-Inositol has been found in millimolar concentrations in dog sciatic nerve and rat kidney. Microgram quantities of TMS-scyllo-inositol have been isolated by gas chromatography from these tissues and identified by infrared comparison with authentic TMS-scyllo-inositol. © 1968, American Chemical Society. All rights reserved.
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页码:819 / &
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