METHYLGLYOXAL-INDUCED DNA-PROTEIN CROSS-LINKS AND CYTO-TOXICITY IN CHINESE-HAMSTER OVARY CELLS

被引：42

作者：

BRAMBILLA, G ^{[1
]}

SCIABA, L ^{[1
]}

FAGGIN, P ^{[1
]}

FINOLLO, R ^{[1
]}

BASSI, AM ^{[1
]}

FERRO, M ^{[1
]}

MARINARI, UM ^{[1
]}

机构：

[1] UNIV GENOA, INST GEN PATHOL, I-16132 GENOA, ITALY

来源：

CARCINOGENESIS | 1985年 / 6卷 / 05期

关键词：

D O I：

10.1093/carcin/6.5.683

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The technique of alkaline elution was applied to study the capacity of methylglyoxal [an antineoplastic drug] to induce DNA damage and repair in Chinese hamster ovary cells. DNA cross-linking was observed after a 90-min exposure to a subtoxic dose (1.5 mM) and the cross-links were fully repaired by 24 h. The cross-linking appeared to be DNA-protein in nature, since proteinase treatment removed the effect. When the same cells were exposed to methylglyoxal in the presence of a rat liver metabolic system, both cytotoxicity and cross-linking frequency were significantly reduced.

引用

页码：683 / 686

页数：4

共 36 条

[1] RAPID ASSAY FOR GLYOXALASE ENZYME SYSTEM [J].