APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO THE STUDY OF THIAMINE METABOLISM AND IN PARTICULAR THIAMINE TRIPHOSPHATASE
被引:11
作者:
BETTENDORFF, L
论文数: 0引用数: 0
h-index: 0
机构:Laboratory of General and Comparative Biochemistry, University of Liège, 4020 Liège
BETTENDORFF, L
机构:
[1] Laboratory of General and Comparative Biochemistry, University of Liège, 4020 Liège
来源:
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS
|
1991年
/
566卷
/
02期
关键词:
D O I:
10.1016/0378-4347(91)80256-C
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Thiamine triphosphate can be found in most tissues at very low levels, but its role is unknown. Organs and muscles that generate electrical impulses are particularly rich in this compound. This paper describes a thiamine triphosphatase from the electrical organ of Electrophorus electricus. The activity of this enzyme, as measured by a high-performance liquid chromatographic method, is closely anion-regulated. Furthermore, thiamine triphosphate increases chloride uptake in membrane vesicles prepared from rat brain. Our results suggest that this compound could play an important role in the regulation of chloride permeability.