PATCH-CLAMP RECORDINGS ON RAT CARDIAC-MUSCLE SLICES

被引:22
作者
BURNASHEV, NA
EDWARDS, FA
VERKHRATSKY, AN
机构
[1] MAX PLANCK INST MED RES,ZELLPHYSIOL ABT,POSTFACH 103820,W-6900 HEIDELBERG 1,GERMANY
[2] AA BOGOMOLETS PHYSIOL INST,KIEV,UKRAINE,USSR
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1990年 / 417卷 / 01期
关键词
clamp-Heart-Cardiac; Patch; slice;
D O I
10.1007/BF00370782
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Thin slices (100-200 μm) from cardiac ventricles of newborn (1-14 days old) rats were used for patch-clamp recordings. High resistance seals (>10 GOhms) could be obtained on this preparation without enzymatic treatment of tissue. Resting potential for cardiac fibres, measured in whole-cell configuration, ranged between -30 and -65 mV. Averaged sodium currents and inward rectifying potassium single-channel currents, recorded in cell-attached mode, displayed similar features to those previously reported for isolated rat ventricular cells. Application of patch-clamp techniques to cardiac slices allows single-channel recordings without complicated procedures of cell isolation. Moreover, possible alteration of channel properties caused by proteolytic enzymes can be avoided. This technique provides a new tool for studying electrophysiological properties of heart cells in situ. © 1990 Springer-Verlag.
引用
收藏
页码:123 / 125
页数:3
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