CHROMATOGRAPHIC PROFILES OF CYANOGEN-BROMIDE FRAGMENTS OF UNREDUCED HUMAN SERUM-ALBUMIN ON IMMOBILIZED CIBACRON BLUE F3G-A

被引：8

作者：

COMPAGNINI, A ^{[1
]}

FICHERA, M ^{[1
]}

FISICHELLA, S ^{[1
]}

FOTI, S ^{[1
]}

SALETTI, R ^{[1
]}

机构：

[1] UNIV CATANIA,DIPARTIMENTO SCI CHIM,V A DORIA 6,I-95125 CATANIA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 639卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80273-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The elution profiles of cyanogen bromide fragments A (299-585), B (1-123), C (124-298) and D (1-298) of unreduced human serum albumin (HSA) on Cibacron Blue F3G-A immobilized on Sepharose CL-6B are reported. The binding properties of fragments C and D are similar to those of HSA, whereas fragment A shows a slightly lower retention time. Fragment B, in contrast, does not interact with the dye. The different chromatographic behaviour of fragments B and C allows their fast separation by combined use of gel permeation and dye-protein affinity chromatography.

引用

页码：341 / 345

页数：5

共 11 条

[1]

BIRKENMEIER G, 1989, PROTEIN DYE INTERACT, P253

[2] HEME BINDING TO HUMAN-SERUM ALBUMIN AND TO 3 LARGE CYANOGEN-BROMIDE ALBUMIN FRAGMENTS [J].

HRKAL, Z ;

KODICEK, M ;

VODRAZKA, Z ;

MELOUN, B ;

MORAVEK, L .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1978, 9 (05) :349-355

[3]

Kopperschlager G., 1982, ADV BIOCHEM ENG, V25, P101

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] ISOLATION AND PROPERTIES OF A FRAGMENT OF HUMAN-SERUM ALBUMIN DEMONSTRATING ABSENCE OF A METHIONINE RESIDUE FROM SOME OF ALBUMIN MOLECULES [J].

LAPRESLE, C ;

DOYEN, N .

BIOCHEMICAL JOURNAL, 1975, 151 (03) :637-643

[6]

MCLOUGHLIN SB, 1988, REV PROG COLORATION, V18, P16, DOI DOI 10.1111/J.1478-4408.1988.TB00063.X

[7]

MCMENAMY RH, 1971, J BIOL CHEM, V246, P4744

[8] STRUCTURE OF N-TERMINAL CYANOGEN-BROMIDE FRAGMENT OF HUMAN PLASMA ALBUMIN [J].

MELOUN, B ;

SABER, MA ;

KUSNIR, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 393 (02) :505-519

[9] DYE-LIGAND AFFINITY-CHROMATOGRAPHY - THE INTERACTION OF CIBACRON BLUE F3GA WITH PROTEINS AND ENZYMES [J].

SUBRAMANIAN, S .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1984, 16 (02) :169-205

[10]

SUBRAMANIAN S, 1989, PROTEIN DYE INTERACT, P56

← 1 2 →