SELECTIVITY OF DNA-POLYMERASES TOWARD ALPHA-NUCLEOTIDE AND BETA-NUCLEOTIDE SUBSTRATES OF D-SERIES AND L-SERIES

被引：29

作者：

SEMIZAROV, DG ^{[1
]}

VICTOROVA, LS ^{[1
]}

DYATKINA, NB ^{[1
]}

VONJANTALIPINSKI, M ^{[1
]}

KRAYEVSKY, AA ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED,D-13125 BERLIN,GERMANY

来源：

FEBS LETTERS | 1994年 / 354卷 / 02期

关键词：

REVERSE TRANSCRIPTASE; TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE; MODIFIED NUCLEOSIDE 5'-TRIPHOSPHATE; STEREOMER;

D O I：

10.1016/0014-5793(94)01123-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The substrate properties of four carbocyclic D and L nucleoside 5'-triphosphate analogs toward HIV and AMV reverse transcriptases and terminal deoxynucleotidyl transferase were evaluated. The compounds of the D-beta and L-beta series were found to be terminating substrates for these enzymes, while the derivatives of the D-alpha and L-alpha. series were recognized only by terminal deoxynucleotidyl transferase, suggesting that for the template-independent enzyme the mutual orientation of the two fragments is of no significance. A hypothesis for binding of nucleotides to the DNA polymerase active center was proposed.

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收藏

页码：187 / 190

页数：4

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