PYRROLIDINE DITHIOCARBAMATE PREVENTS IL-1-INDUCED NITRIC-OXIDE SYNTHASE MESSENGER-RNA, BUT NOT SUPEROXIDE-DISMUTASE MESSENGER-RNA, IN INSULIN-PRODUCING CELLS

被引：54

作者：

BEDOYA, FJ ^{[1
]}

FLODSTROM, M ^{[1
]}

EIZIRIK, DL ^{[1
]}

机构：

[1] UNIV UPPSALA,BIOMEDICUM,DEPT MED CELL BIOL,S-75123 UPPSALA,SWEDEN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1731

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We presently investigated the effects of pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of nuclear factor kappa B (NF-kappa B), on the induction of nitric oxide synthase (iNOS) and manganese superoxide dismutase (MnSOD) mRNAs by IL-1 beta in insulin-producing RIN cells. PDTC decreased by 90% both IL-1 beta-induced increase in medium nitrite accumulation (an indicator of NO production) and induction of iNOS mRNA expression. However, PDTC did not prevent induction of MnSOD mRNA by IL-1 beta. PDTC induced an early (45 min) expression of c-fos mRNA and potentiated IL-1 beta-induced c-fos expression. Our data suggest that NF-kappa B activation is necessary for iNOS, but not for MnSOD, mRNA expression in insulin producing cells. (C) 1995 Academic Press. Inc.

引用

页码：816 / 822

页数：7

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