PRODUCTION OF BRASSICA-NAPUS BY RAPHANOBRASSICA HYBRIDS BY EMBRYO RESCUE - AN ATTEMPT TO INTRODUCE SHATTERING RESISTANCE INTO BRASSICA-NAPUS

被引：13

作者：

AGNIHOTRI, A ^{[1
]}

SHIVANNA, KR ^{[1
]}

RAINA, SN ^{[1
]}

LAKSHMIKUMARAN, M ^{[1
]}

PRAKASH, S ^{[1
]}

JAGANNATHAN, V ^{[1
]}

机构：

[1] TATA ENERGY RES INST,DIV BIOTECHNOL,90 JOHR BAGH,NEW DELHI 110003,INDIA

来源：

PLANT BREEDING | 1990年 / 105卷 / 04期

关键词：

BRASSICA-NAPUS; RAPHANOBRASSICA; WIDE HYBRIDIZATION; EMBRYO RESCUE; DNA ANALYSIS; SHATTERING RESISTANCE; BACKCROSSING;

D O I：

10.1111/j.1439-0523.1990.tb01288.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Intergeneric hybridization is one of the effective methods to broaden the genetic base of cultivated species. Raphanus sativus, a member of the subtribe Raphaninae, has very hard pods at threshing time. In an attempt to transfer shattering resistance to Brassica napus, Raphanobrassica was used as the male parent in crosses with Brassica napus. Plantlets were obtained by embryo rescue and were further multiplied in vitro by micropropagation of nodal segments. Morphology, cytology and DNA analysis confirmed the hybrid nature of these plants. They were backcrossed with Brassica napus and the progeny was raised. Plants of BC1 and BC2 generations showed wide variation in morphology, chromosome number and pollen fertility. Some of the plants showed up to 95% pollen fertility and resistance to shattering, indicating the potential for developing B. napus with resistance to shattering.

引用

页码：292 / 299

页数：8

共 13 条

[1] PRODUCTION OF ERUCA-BRASSICA HYBRIDS BY EMBRYO RESCUE [J].

AGNIHOTRI, A ;

GUPTA, V ;

LAKSHMIKUMARAN, MS ;

SHIVANNA, KR ;

PRAKASH, S ;

JAGANNATHAN, V .

PLANT BREEDING, 1990, 104 (04) :281-289

[2]

BATRA V, 1989, 6 P INT C SABRAO TSU, P443

[3]

Dellaporta S.L., 1984, MOL BIOL PLANTS LABO, P36

[4] MATROMORPHY IN BRASSICA-OLERACEA L .1. TERMINOLOGY, PARTHENOGENESIS IN CRUCIFERAE AND FORMATION AND USABILITY OF MATROMORPHIC PLANTS [J].

EENINK, AH .

EUPHYTICA, 1974, 23 (02) :429-433

[5]

Kumar P. B. A. N., 1988, Sexual Plant Reproduction, V1, P234

[6] ISOLATION AND CHARACTERIZATION OF A HIGHLY REPETITIVE DNA OF BRASSICA-CAMPESTRIS [J].

LAKSHMIKUMARAN, M ;

RANADE, SA .

PLANT MOLECULAR BIOLOGY, 1990, 14 (03) :447-448

[7] LONG INTERSPERSED REPEATED DNA (LINE) CAUSES POLYMORPHISM AT THE RAT INSULIN-1 LOCUS [J].

LAKSHMIKUMARAN, MS ;

DAMBROSIO, E ;

LAIMINS, LA ;

LIN, DT ;

FURANO, AV .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (09) :2197-2203

[8] UBER EINE SPEZIFISCHE ANFARBUNG DER POLLENSCHLAUCHE IM GRIFFEL UND DIE ZAHL DER KALLOSEPFROPFEN NACH SELBSTUNG UND FREMDUNG [J].

LINSKENS, HF ;

ESSER, K .

NATURWISSENSCHAFTEN, 1957, 44 (01) :16-16

[9]

MCNAUGHTON IH, 1978, ANN REPORT SCOTTISH, P75

[10]

Mizushima U., 1980, Brassica crops and wild allies. [I]., P89

← 1 2 →