RECOMBINANT IMMUNOBLOT ASSAY 1ST AND 2ND GENERATIONS ON 732 BLOOD-DONORS REACTIVE FOR ANTIBODIES TO HEPATITIS-C VIRUS BY ELISA

被引：15

作者：

COUROUCE, AM ^{[1
]}

JANOT, C ^{[1
]}

BOUDART, D ^{[1
]}

COTTE, C ^{[1
]}

ELGHOUZZI, MH ^{[1
]}

JULLIEN, AM ^{[1
]}

LEMAIRE, JM ^{[1
]}

MAISONNEUVE, P ^{[1
]}

MANIEZ, M ^{[1
]}

MATTLINGER, B ^{[1
]}

MENAULT, M ^{[1
]}

MESNIER, F ^{[1
]}

MONTCHARMONT, P ^{[1
]}

MOUILLOT, L ^{[1
]}

NORTH, ML ^{[1
]}

OPOLON, P ^{[1
]}

SMILOVICI, W ^{[1
]}

TREPO, C ^{[1
]}

机构：

[1] CTR REG TRANSFUS SANGUINE & HEMATOL,VANDOEUVRE NANCY,FRANCE

来源：

VOX SANGUINIS | 1991年 / 61卷 / 03期

关键词：

D O I：

10.1111/j.1423-0410.1991.tb00943.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A comparison between recombinant immunoblot assay hepatitis C virus (HCV) first generation (RIBA-1) and second generation (RIBA-2) was made on 732 blood donors reactive by anti-HCV ELISA (Ortho Diagnostics System) by the Hepatitis Study Group of the French Society of Blood Transfusion. RIBA-2 results were correlated with ELISA ratio and ALT levels. The number of both reactive and nonreactive samples was higher with RIBA-2 than with RIBA-1, 252 (34%) compared to 224 (31%) for reactive samples, and 404 (55%) compared to 307 (42%) for nonreactive samples. C 22-3 and C 33-c reactivities were observed in 96 and 91% of the reactive samples, respectively. A total of 76 samples (11%) remained indeterminate by RIBA-2, 84% of them reacting only on C 100-3 antigen. A clear relationship between RIBA-2 results and both ELISA ratio and alanine aminotransferase (ALT) levels was demonstrated: 20% of samples with normal ALT level and 10% of samples with low ELISA ratio were reactive when 91% of samples with ALT > 2N and 69% of samples with high ELISA ratio were reactive. The totality of the 57 samples with both ALT > 2N and high ELISA ratio were reactive and 93% of samples with normal ALT level and low ELISA ratio were nonreactive.

引用

页码：177 / 180

页数：4

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