SELECTIVITY IN ELECTROPHORETICALLY MEDIATED MICROANALYSIS BY CONTROL OF PRODUCT DETECTION TIME

被引:42
作者
HARMON, BJ [1 ]
LEESONG, I [1 ]
REGNIER, FE [1 ]
机构
[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907
关键词
D O I
10.1021/ac00093a041
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Differential electrophoretic mobility between an analyte and its product offers electrophoretically mediated microanalysis (EMMA) a unique capability to selectively control the detection time of the product of a reaction-based chemical analysis. If an analyte and its product differ in migration velocity under the influence of an applied electric field, the apparent velocity and, consequently, the detection time of the product are dependent upon the relative amounts of time the product effectively traverses the capillary with the respective mobilities of the analyte and the product. Consequently, by controlling when the analytical reaction is allowed to occur, the detection time of the reaction product can be selectively maneuvered within a time window defined by the mobilities of the analyte and the product. This paper describes the use of this technique to manipulate the detection time of product profiles independently of nonreacting matrix interferants for the determination of both enzymes and substrates by EMMA. Furthermore, the ability to manipulate product detection times allows for simultaneous EMILIA determinations of multiple enzymes or substrates.
引用
收藏
页码:3797 / 3805
页数:9
相关论文
共 9 条
[1]   CATALYTIC ACTIVITY OF NATIVE ENZYMES DURING CAPILLARY ELECTROPHORESIS - AN ENZYMATIC MICROREACTOR [J].
AVILA, LZ ;
WHITESIDES, GM .
JOURNAL OF ORGANIC CHEMISTRY, 1993, 58 (20) :5508-5512
[2]   ULTRAMICRO ENZYME ASSAYS IN A CAPILLARY ELECTROPHORETIC SYSTEM [J].
BAO, JM ;
REGNIER, FE .
JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :217-224
[3]   MATHEMATICAL TREATMENT OF ELECTROPHORETICALLY MEDIATED MICROANALYSIS [J].
HARMON, BJ ;
PATTERSON, DH ;
REGNIER, FE .
ANALYTICAL CHEMISTRY, 1993, 65 (19) :2655-2662
[4]   ELECTROPHORETICALLY MEDIATED MICROANALYSIS OF ETHANOL [J].
HARMON, BJ ;
PATTERSON, DH ;
REGNIER, FE .
JOURNAL OF CHROMATOGRAPHY A, 1993, 657 (02) :429-434
[5]   FLOW-INJECTION ANALYSIS IN THE CAPILLARY FORMAT USING ELECTROOSMOTIC PUMPING [J].
LIU, SR ;
DASGUPTA, PK .
ANALYTICA CHIMICA ACTA, 1992, 268 (01) :1-6
[6]   ELECTROPHORETICALLY MEDIATED MICROANALYSIS OF LEUCINE AMINOPEPTIDASE IN COMPLEX MATRICES USING TIME-RESOLVED LASER-INDUCED FLUORESCENCE DETECTION [J].
MILLER, KJ ;
LEESONG, IK ;
BAO, JM ;
REGNIER, FE ;
LYTLE, FE .
ANALYTICAL CHEMISTRY, 1993, 65 (22) :3267-3270
[7]   ELECTROPHORETICALLY MEDIATED MICROANALYSIS OF CALCIUM [J].
PATTERSON, DH ;
HARMON, BJ ;
REGNIER, FE .
JOURNAL OF CHROMATOGRAPHY A, 1994, 662 (02) :389-395
[8]   NATIVE PROTEIN SEPARATIONS AND ENZYME MICROASSAYS BY CAPILLARY ZONE AND GEL-ELECTROPHORESIS [J].
WU, D ;
REGNIER, FE .
ANALYTICAL CHEMISTRY, 1993, 65 (15) :2029-2035
[9]   VARIABILITY OF INTRACELLULAR LACTATE-DEHYDROGENASE ISOENZYMES IN SINGLE HUMAN ERYTHROCYTES [J].
XUE, QF ;
YEUNG, ES .
ANALYTICAL CHEMISTRY, 1994, 66 (07) :1175-1178