EXPRESSION OF THE BACILLUS-SUBTILIS DINR AND RECA GENES AFTER DNA DAMAGE AND DURING COMPETENCE

被引:27
作者
RAYMONDDENISE, A [1 ]
GUILLEN, N [1 ]
机构
[1] INST PASTEUR,INSERM,U199,UNITE PATHOGENIE MICROBIENNE MOLEC,F-75724 PARIS 15,FRANCE
关键词
D O I
10.1128/jb.174.10.3171-3176.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Bacillus subtilis dinR gene product is homologous to the LexA protein of Escherichia coli and regulates the expression of dinR and dinC. Using transcriptional fusions in the dinR and the recA genes, we have investigated the epistatic relationship between these two genes during the SOS response induced either by DNA damage or by competence. The results show that after DNA damage, induction of the expression of both recA and dinR is dependent on the activity of the DinR and RecA proteins. A RecA-dependent activity on DinR is proposed as the initial event in the induction of the SOS network. In contrast, the competence-related induction of dinR and recA appears to involve two distinct mechanisms. While one mechanism corresponds to the classical regulation of the SOS response, the other appears to involve an activating factor. Moreover, this factor is active in cells in which competence is prevented by a mutation in the regulatory gene comA.
引用
收藏
页码:3171 / 3176
页数:6
相关论文
共 27 条
[1]   EXPRESSION OF COMPETENCE GENES IN BACILLUS-SUBTILIS [J].
ALBANO, M ;
HAHN, J ;
DUBNAU, D .
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) :3110-3117
[2]  
CHEN NY, 1989, J GEN MICROBIOL, V135, P2931
[3]   CLONING AND CHARACTERIZATION OF DNA DAMAGE-INDUCIBLE PROMOTER REGIONS FROM BACILLUS-SUBTILIS [J].
CHEO, DL ;
BAYLES, KW ;
YASBIN, RE .
JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1696-1703
[4]   GENETIC COMPETENCE IN BACILLUS-SUBTILIS [J].
DUBNAU, D .
MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :395-424
[5]   DNA-REPAIR IN B-SUBTILIS - AN INDUCIBLE DIMER SPECIFIC W-REACTIVATION SYSTEM [J].
FIELDS, PI ;
YASBIN, RE .
MOLECULAR AND GENERAL GENETICS, 1983, 190 (03) :475-480
[6]  
FTOUHI N, 1990, GENETICS, V126, P487
[7]   EXPRESSION OF THE RECE GENE DURING INDUCTION OF THE SOS RESPONSE IN BACILLUS-SUBTILIS RECOMBINATION-DEFICIENT STRAINS [J].
GASSEL, M ;
ALONSO, JC .
MOLECULAR MICROBIOLOGY, 1989, 3 (09) :1269-1276
[8]   INDUCTION OF MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN-B RESISTANCE REQUIRES RIBOSOMES ABLE TO BIND INDUCER [J].
GRYCZAN, TJ ;
ISRAELIRECHES, M ;
DUBNAU, D .
MOLECULAR & GENERAL GENETICS, 1984, 194 (03) :357-361
[9]   CLONING AND CHARACTERIZATION OF THE REGULATORY BACILLUS-SUBTILIS COMPETENCE GENES COMA AND COMB [J].
GUILLEN, N ;
WEINRAUCH, Y ;
DUBNAU, DA .
JOURNAL OF BACTERIOLOGY, 1989, 171 (10) :5354-5361
[10]   CHROMOSOMAL LOCATION AND PROPERTIES OF RADIATION SENSITIVITY MUTATIONS IN BACILLUS-SUBTILIS [J].
HOCH, JA ;
ANAGNOSTOPOULOS, C .
JOURNAL OF BACTERIOLOGY, 1970, 103 (02) :295-+