PHARMACOLOGICAL EVIDENCE FOR NEUROKININ RECEPTORS IN MURINE NEUROBLASTOMA C1300 CELLS

被引：11

作者：

FUKUHARA, S ^{[1
]}

MUKAI, H ^{[1
]}

MUNEKATA, E ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

来源：

PEPTIDES | 1995年 / 16卷 / 02期

关键词：

TACHYKININ; NEUROKININ A; NEUROKININ B; RECEPTOR; INTRACELLULAR FREE CA2+; C1300; CELLS;

D O I：

10.1016/0196-9781(94)00166-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that neurokinin A (NKA) and neurokinin B (NKB) induce an increase in the concentration of intracellular free Ca2+ ([Ca2+](i)) in murine neuroblastoma C1300 cells (EC(50): NKA 87 +/- 13 nM, NKB 97 +/- 15 nM). Substance P (SP) also caused a transient Ca2+ increase, although the potency of SP was much less than that of NKA and NKB. The increase in [Ca2+](i) induced by NKA and NKB was inhibited by SR 48,968, a selective antagonist for NK2, and [beta Ala(8)]NKA(4-10), a selective agonist for NK2, did not stimulate the increase in [Ca2+](i). NKA- and NKB-induced Ca2+ mobilization was not inhibited by CP-96,345 and [Trp(7),beta Ala(8)]NKA(4-10), selective antagonists for NK1 and NK3, respectively. These results suggested that C1300 cells express endogenous NK2 neurokinin receptors that have different: features from known NK2 receptors.

引用

页码：211 / 214

页数：4

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