THE T-PROTEIN ENCODED BY BRACHYURY IS A TISSUE-SPECIFIC TRANSCRIPTION FACTOR

被引:251
作者
KISPERT, A [1 ]
KOSCHORZ, B [1 ]
HERRMANN, BG [1 ]
机构
[1] MAX PLANCK INST ENTWICKLUNGSBIOL,BIOCHEM ABT,D-72076 TUBINGEN,GERMANY
关键词
BRACHYURY; EMBRYOGENESIS; MESODERM; MOUSE; TRANSCRIPTION FACTOR;
D O I
10.1002/j.1460-2075.1995.tb00158.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mouse Brachyury (T) gene is required for differentiation of the notochord and formation of mesoderm during posterior development. Homozygous embryos lacking T activity do not develop a trunk and tail and die in utero. The T gene is specifically expressed in notochord and early mesoderm cells in the embryo. Recent data have demonstrated that the T protein is localized in the cell nucleus and specifically binds to a palindrome of 20 bp (the T site) in vitro. We show that the T protein activates expression of a reporter gene in HeLa cells through binding to the T site, Thus T is a novel tissue-specific transcription factor, It consists of a large N-terminal DNA binding domain (amino acids 1-229) and two pairs of transactivation and repression domains in the C-terminal protein half, T can also transactivate transcription through variously oriented and spaced T sites, a fact that may be relevant in the search for genes controlled by T protein and important in mesoderm development.
引用
收藏
页码:4763 / 4772
页数:10
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