RAPID PURIFICATION OF A CLONED GENE-PRODUCT BY GENETIC FUSION AND SITE-SPECIFIC PROTEOLYSIS

被引:157
作者
GERMINO, J [1 ]
BASTIA, D [1 ]
机构
[1] DUKE UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,DURHAM,NC 27710
来源
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES | 1984年 / 81卷 / 15期
关键词
D O I
10.1073/pnas.81.15.4692
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
引用
收藏
页码:4692 / 4696
页数:5
相关论文
共 19 条
[1]   MOLECULAR-STRUCTURE OF ELONGATED FORMS OF ELECTRIC-EEL ACETYLCHOLINESTERASE [J].
ANGLISTER, L ;
SILMAN, I .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 125 (03) :293-311
[2]   SEQUENCE DETERMINATION AND ANALYSIS OF THE 3' REGION OF CHICKEN PRO-ALPHA-1(I) AND PRO-ALPHA-2(I) COLLAGEN MESSENGER RIBONUCLEIC-ACIDS INCLUDING THE CARBOXY-TERMINAL PROPEPTIDE SEQUENCES [J].
FULLER, F ;
BOEDTKER, H .
BIOCHEMISTRY, 1981, 20 (04) :996-1006
[3]   INTERACTION OF THE PLASMID R6K-ENCODED REPLICATION INITIATOR PROTEIN WITH ITS BINDING-SITES ON DNA [J].
GERMINO, J ;
BASTIA, D .
CELL, 1983, 34 (01) :125-134
[4]   PRIMARY STRUCTURE OF THE REPLICATION INITIATION PROTEIN OF PLASMID R6K [J].
GERMINO, J ;
BASTIA, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (18) :5475-5479
[5]   THE REPLICATION INITIATOR PROTEIN OF PLASMID-R6K TAGGED WITH BETA-GALACTOSIDASE SHOWS SEQUENCE-SPECIFIC DNA-BINDING [J].
GERMINO, J ;
BASTIA, D .
CELL, 1983, 32 (01) :131-140
[6]   USE OF GENE FUSIONS AND PROTEIN PROTEIN-INTERACTION IN THE ISOLATION OF A BIOLOGICALLY-ACTIVE REGULATORY PROTEIN - THE REPLICATION INITIATOR PROTEIN OF PLASMID-R6K [J].
GERMINO, J ;
GRAY, JG ;
CHARBONNEAU, H ;
VANAMAN, T ;
BASTIA, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (22) :6848-6852
[7]   IMPROVED METHODS FOR MAXIMIZING EXPRESSION OF A CLONED GENE - A BACTERIUM THAT SYNTHESIZES RABBIT BETA-GLOBIN [J].
GUARENTE, L ;
LAUER, G ;
ROBERTS, TM ;
PTASHNE, M .
CELL, 1980, 20 (02) :543-553
[8]  
HARPER E, 1970, COLLAGENASE, P19
[9]  
MULLERHILL, 1974, NATURE, V249, P561
[10]   AMINO-TERMINAL FRAGMENT OF LAC REPRESSOR BINDS SPECIFICALLY TO LAC OPERATOR [J].
OGATA, RT ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (12) :5851-5854