THE USE OF CHARGE-COUPLED-DEVICES IN THE QUANTITATIVE-EVALUATION OF IMAGES, ON PHOTOGRAPHIC FILM OR MEMBRANES, OBTAINED FOLLOWING ELECTROPHORETIC SEPARATION OF DNA FRAGMENTS

被引：20

作者：

BONISZEWSKI, ZAM ^{[1
]}

COMLEY, JS ^{[1
]}

HUGHES, B ^{[1
]}

READ, CA ^{[1
]}

机构：

[1] AMERSHAM INT PLC,AMERSHAM PL,LITTLE CHALFONT HP7 9NA,BUCKS,ENGLAND

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 05期

关键词：

D O I：

10.1002/elps.1150110514

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Charge‐coupled devices can provide an excellent means for the quantification of DNA band images on membranes and photographic film. However, proper consideration must be given to important parameters such as signal‐to‐noise ratio and resolution, especially in applications which demand the use of large format media. When employed in the direct imaging of chemiluminescent blots, the charge‐coupled device can provide equal or better sensitivity than that obtained by indirect methods using film, with the additional advantages of wide dynamic range and freedom from the vagaries of film processing. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：432 / 440

页数：9

共 23 条

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