CRITICAL-EVALUATION OF CYTOSOLIC CALCIUM DETERMINATION IN RESTING MUSCLE-FIBERS FROM NORMAL AND DYSTROPHIC (MDX) MICE

被引：102

作者：

GAILLY, P ^{[1
]}

BOLAND, B ^{[1
]}

HIMPENS, B ^{[1
]}

CASTEELS, R ^{[1
]}

GILLIS, JM ^{[1
]}

机构：

[1] KUL, PHYSIOL LAB, LOUVAIN, BELGIUM

来源：

CELL CALCIUM | 1993年 / 14卷 / 06期

关键词：

D O I：

10.1016/0143-4160(93)90006-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The fluorescent probe Fura-2/AM was used to determine cytosolic free calcium concentration in soleus muscle and in Isolated flexor digitorum brevis fibres. This required a precise calibration; therefore, each calibration parameter was studied in situ. The influence of the dye concentration on calcium measurements was also examined. This precise calibration technique was used to compare absolute free calcium concentration in resting preparations from dystrophic (mdx) and control (C57) mice. We showed that the behavior of the dye was not similar in C57 and in mdx muscles. For this reason, we did not confirm the previous results that cytosolic free calcium concentration is increased in mdx muscles.

引用

页码：473 / 483

页数：11

共 43 条

[1] THE CA SIGNAL FROM FURA-2 LOADED MAST-CELLS DEPENDS STRONGLY ON THE METHOD OF DYE-LOADING [J].