MUTATIONAL ANALYSIS OF THE PSEUDOKNOT STRUCTURE OF THE S15 TRANSLATIONAL OPERATOR FROM ESCHERICHIA-COLI

被引：23

作者：

BENARD, L

PHILIPPE, C

DONDON, L

GRUNBERGMANAGO, M

EHRESMANN, B

EHRESMANN, C

PORTIER, C

机构：

[1] INST BIOL PHYS CHIM, F-75005 PARIS, FRANCE

[2] CNRS, INST BIOL MOLEC & CELLULAIRE, F-67084 STRASBOURG, FRANCE

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb01264.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of rpsO the gene encoding the small ribosomal protein S15, is autoregulated at the translational level by S15, which binds to its mRNA in a region overlapping the ribosome-binding site. By measuring the effect of mutations on the expression of a translational rpsO-lacZ fusion and the S15 binding affinity for the translational operator, the formation of a pseudoknot in the operator site in vivo is fully demonstrated and appears to be a prerequisite for S15 binding. The mutational analysis suggests also that specific determinants for S15 binding are located in very limited regions of the structure formed by the pseudoknot. It is deduced that a specific pseudoknot conformation is a key element for autoregulation.

引用

页码：31 / 40

页数：10

共 41 条

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