FLUORESCENCE POLARIZATION ASSAY FOR ENDOTHELIN-CONVERTING ENZYMES

被引：5

作者：

HAZAN, S

FISHOV, I

ZAMAI, M

CAIOLFA, VR

PAROLA, AH

机构：

[1] BEN GURION UNIV NEGEV,DEPT CHEM,IL-84105 BEER SHEVA,ISRAEL

[2] BEN GURION UNIV NEGEV,DEPT LIFE SCI,IL-84105 BEER SHEVA,ISRAEL

[3] PHARMACIA FARMITALIA,RES LABS,ONCOL BUSINESS AREA,I-20014 NERVIANO,ITALY

来源：

PEPTIDES | 1995年 / 16卷 / 05期

关键词：

ENDOTHELIN; TRYPTOPHAN FLUORESCENCE; FLUORESCENCE POLARIZATION; ENDOTHELIN-CONVERTING ENZYME ASSAY;

D O I：

10.1016/0196-9781(95)00032-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of big endothelin to endothelin by alpha-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

引用

页码：833 / 836

页数：4

共 14 条

[1]

BEECHEM JM, 1985, ANNU REV BIOCHEM, V54, P43, DOI 10.1146/annurev.biochem.54.1.43

[2]

DOHERTY AM, 1992, J MED CHEM, V39, P1493

[3]

FIRTH JD, 1988, LANCET, P1179

[4] THE OBLIGATORY ROLE OF ENDOTHELIAL-CELLS IN THE RELAXATION OF ARTERIAL SMOOTH-MUSCLE BY ACETYLCHOLINE [J].