CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF THE VL DOMAIN OF THE ANTIBODY MCPC603 PRODUCED IN ESCHERICHIA-COLI

被引：25

作者：

GLOCKSHUBER, R

STEIPE, B

HUBER, R

PLUCKTHUN, A

机构：

[1] UNIV MUNICH, GENZENTRUM, W-8033 MARTINSRIED, GERMANY

[2] MAX PLANCK INST BIOCHEM, STRUKTURFORSCH ABT, W-8033 MARTINSRIED, GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 213卷 / 04期

关键词：

D O I：

10.1016/S0022-2836(05)80247-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The VL domain, obtained from a recombinant FV fragment of the antibody McPC603 expressed in Escherichia coli, has been crystallized as a dimer from 2 m-NH4)2SO4 (pH 4·0). The crystals are hexagonal, space group P6122. The cell dimensions are a=b=86·48 Å, c=76·64 Å, with a VL monomer as the asymmetric unit. The crystals diffract to 2·0 Å. The structure was solved by Patterson search using the VL domain of the Fab fragment of McPC603 and the VL dimer REI. © 1990 Academic Press Limited.

引用

收藏

页码：613 / 615

页数：3

相关论文

共 21 条

[1]

ALZARI PM, 1988, ANNU REV IMMUNOL, V6, P555

[2] CRYSTAL AND MOLECULAR-STRUCTURE OF DIMER OF VARIABLE DOMAINS OF BENCE-JONES PROTEIN ROY [J].

COLMAN, PM ;

SCHRAMM, HJ ;

GUSS, JM .

JOURNAL OF MOLECULAR BIOLOGY, 1977, 116 (01) :73-79

[3] A METHOD OF POSITIONING A KNOWN MOLECULE IN AN UNKNOWN CRYSTAL STRUCTURE [J].

CROWTHER, RA ;

BLOW, DM .

ACTA CRYSTALLOGRAPHICA, 1967, 23 :544-&

[4] MOLECULAR-STRUCTURE OF A DIMER COMPOSED OF VARIABLE PORTIONS OF BENCE-JONES PROTEIN REI REFINED AT 2.0-A RESOLUTION [J].

EPP, O ;

LATTMAN, EE ;

SCHIFFER, M ;

HUBER, R ;

PALM, W .

BIOCHEMISTRY, 1975, 14 (22) :4943-4952

[5] STRUCTURE DETERMINATION OF VARIABLE PORTION OF BENCE-JONES PROTEIN AU [J].

FEHLHAMMER, H ;

SCHIFFER, M ;

EPP, O ;

COLMAN, PM ;

LATTMAN, EE ;

SCHWAGER, P ;

STEIGEMANN, W ;

SCHRAMM, HJ .

BIOPHYSICS OF STRUCTURE AND MECHANISM, 1975, 1 (02) :139-146

[6] STRUCTURE OF A NOVEL BENCE-JONES PROTEIN (RHE) FRAGMENT AT 1.6 A RESOLUTION [J].

FUREY, W ;

WANG, BC ;

YOO, CS ;

SAX, M .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 167 (03) :661-692

[7] A COMPARISON OF STRATEGIES TO STABILIZE IMMUNOGLOBULIN FV-FRAGMENTS [J].

GLOCKSHUBER, R ;

MALIA, M ;

PFITZINGER, I ;

PLUCKTHUN, A .

BIOCHEMISTRY, 1990, 29 (06) :1362-1367

[8] EXPERIMENTAL ABSORPTION CORRECTION - RESULTS [J].

HUBER, R ;

KOPFMANN, G .

ACTA CRYSTALLOGRAPHICA SECTION A-CRYSTAL PHYSICS DIFFRACTION THEORETICAL AND GENERAL CRYSTALLOGRAPHY, 1969, A 25 :143-&

[9]

HUBER R, 1969, CRYSTALLOGRAPHIC COM, P96

[10] CRYSTAL ORIENTATION AND X-RAY PATTERN PREDICTION ROUTINES FOR AREA-DETECTOR DIFFRACTOMETER SYSTEMS IN MACROMOLECULAR CRYSTALLOGRAPHY [J].

MESSERSCHMIDT, A ;

PFLUGRATH, JW .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1987, 20 (04) :306-315