NONVIRAL HETEROGENEOUS SEQUENCES AT THE 5' ENDS OF TOMATO SPOTTED WILT VIRUS MESSENGER-RNAS

被引：52

作者：

KORMELINK, R

VANPOELWIJK, F

PETERS, D

GOLDBACH, R

机构：

[1] Department of Virology, 6709 PD Wageningen

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-8-2125

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Subgenomic messenger RNAs transcribed from the tomato spotted wilt virus (TSWV) S RNA segment were partially purified from total RNA extracts of TSWV-infected Nicotiana rustica and analysed by primer extension analysis. The data obtained show the presence of non-viral sequences, 12 to 20 nucleotides in length, at the 5' ends of the N and NS(S) mRNAs, indicating a cap-snatching mechanism for the initiation of transcription. This is the first report of a plant virus using such a mechanism for transcription of the viral genome.

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页码：2125 / 2128

页数：4

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