DETECTION OF POLY(ADP RIBOSE) POLYMERASE IN CRUDE EXTRACTS BY ACTIVITY-BLOT

被引：65

作者：

SIMONIN, F

BRIAND, JP

MULLER, S

DEMURCIA, G

机构：

[1] UNIV STRASBOURG 1,CNRS,INST BIOL MOLEC & CELLULAIRE,BIOCHIM LAB 2,15 RUE RENE DESCARTES,F-67084 STRASBOURG,FRANCE

[2] UNIV STRASBOURG 1,CTR RECH,BIOCHIM LAB 2,F-67084 STRASBOURG,FRANCE

[3] UNIV STRASBOURG 1,IMMUNOCHIM LAB,F-67084 STRASBOURG,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90321-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently devised an activity-blot procedure permitting the detection, on the same nitrocellulose sheet, of the functional poly(ADP ribose) polymerase (PARP) activity as well as the immunostained active peptide(s) after renaturation of the transferred protein(s). Using this technique we have analyzed the PARP activity in higher and lower eukaryotes directly on crude extracts from cell cultures. This procedure has been extended also to in situ screening of bacterial colonies expressing the PARP enzymatic activity. © 1991.

引用

页码：226 / 231

页数：6

共 30 条

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