SUBZONAL SPERM INSERTION AND THE FREQUENCY OF GAMETE FUSION

被引：8

作者：

ALIKANI, M ^{[1
]}

ADLER, A ^{[1
]}

REING, AM ^{[1
]}

MALTER, HE ^{[1
]}

COHEN, J ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,NEW YORK HOSP,DEPT OBSTET & GYNECOL,CTR REPROD MED & INFERTIL,NEW YORK,NY 10021

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 02期

关键词：

SUBZONAL SPERM INSERTION; NUMBER OF MALE PRONUCLEI; POLYSPERMY;

D O I：

10.1007/BF01203746

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The subzonal sperm insertion technique was applied to assess the potential of motile human spermatozoa to form pronuclei. In 184 mature human oocytes, subzonal sperm insertion was used as the primary mode of insemination in cases with abnormal semen analyses. Oocytes (n = 131) that failed to fertilize in vitro in cases with normal semen profiles were also micromanipulated for secondary insemination. The frequency of sperm fusion, expressed as a percentage, was defined as the total number of male pronuclei formed divided by the total number of spermatozoa inserted subzonally. Our results indicate that 37% of spermatozoa from men with normal semen are able to fuse with the oolemma and decondense within the ooplasm, when placed in the perivitelline space of the oocyte. Excluding the oocytes that appeared morphologically abnormal (presence of cytoplasmic inclusions such as refractile bodies within the ooplasm), the frequency of sperm fusion increased to nearly 60%. Moreover, 14% of subzonally inserted spermatozoa from men with abnormal semen analyses demonstrated an ability to form a pronucleus. The incidence of polyspermy was high, ranging from 30 to 80% in the different groups studied. It is therefore concluded that the human oolemma provides little protection against multiple sperm fusion and that the frequency of gamete fusion is unexpectedly high, even when the spermatozoa are derived from infertile men.

引用

页码：97 / 101

页数：5

共 20 条

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