ROLE OF ACIDIC COMPARTMENTS IN TRYPANOSOMA-BRUCEI, WITH SPECIAL REFERENCE TO LOW-DENSITY-LIPOPROTEIN PROCESSING

被引:43
作者
COPPENS, I
BAUDHUIN, P
OPPERDOES, FR
COURTOY, PJ
机构
[1] UNIV CATHOLIQUE LOUVAIN, SCH MED, CELL BIOL UNIT, B-1200 BRUSSELS, BELGIUM
[2] INT INST CELLULAR & MOLEC PATHOL, TROP DIS RES UNIT, B-1200 BRUSSELS, BELGIUM
关键词
TRYPANOSOMA-BRUCEI; ENDOCYTOSIS; LDL PROTEOLYSIS; LDL RECEPTOR RECYCLING; CHLOROQUINE; MONENSIN;
D O I
10.1016/0166-6851(93)90044-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the bloodstream form of Trypanosoma brucei, specific receptors mediate the endocytosis of host low-density lipoprotein particles. We have explored the fate of ligand and receptor with a biochemical approach, using inhibitors of the endocytotic apparatus. The weak base chloroquine rapidly accumulates in trypanosomes, its uptake is prevented by the proton ionophore monensin, and it induces the swelling of intracellular vacuoles, indicating that their content is acidic. Cell-associated LDL is rapidly degraded into intermediately sized fragments and TCA-soluble material that can be recovered in cell extracts and extracellular medium. Chloroquine, leupeptin and E64, but not PMSF, efficiently prevent LDL proteolysis, suggesting that degradation occurs in those acidic compartment(s) and is mediated by thiol-protease(s). Both monensin and chloroquine decrease the number of LDL receptors exposed at the cell surface, a phenomenon amplified in the presence of LDL. This provides indirect evidence that internalised LDL receptors are recycled at a rate which is slowed down by receptor occupancy and by agents that impair acidification of the endocytic organelles. Finally, chloroquine decreases by half the growth rate of procyclic trypanosomes in vitro at 5 mug ml-1. At 40 mg kg-1 per day, it also slows down the increase in parasitaemia and prolongs the survival time of infected mice by up to 2 days.
引用
收藏
页码:223 / 232
页数:10
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