ASCARIS-SUUM - CLONING OF A CDNA-ENCODING PHOSPHOENOLPYRUVATE CARBOXYKINASE

被引：16

作者：

GEARY, TG ^{[1
]}

WINTERROWD, CA ^{[1
]}

ALEXANDERBOWMAN, SJ ^{[1
]}

FAVREAU, MA ^{[1
]}

NULF, SC ^{[1
]}

KLEIN, RD ^{[1
]}

机构：

[1] UPJOHN CO,UPJOHN LABS,MOLEC BIOL RES,KALAMAZOO,MI 49001

来源：

EXPERIMENTAL PARASITOLOGY | 1993年 / 77卷 / 02期

关键词：

ASCARIS-SUUM (A-SUUM); NEMATODE; PARASITE; EC; 4.1.1.32;

D O I：

10.1006/expr.1993.1072

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A cDNA encoding phosphoenolpyruvate carboxykinase (PEPCK) from Ascaris suum was cloned by complementation of a strain of Escherichia coli deficient in PEPCK and malic enzyme. The product of this cDNA was enzymatically similar to a recombinant PEPCK obtained from Haemonchus contortus by the same method. Comparison of the predicted amino acid sequence of A. suum PEPCK with other PEPCKs showed that this enzyme is most closely related to the H. contortus enzyme. The two nematode enzymes share considerable homology in regions thought to be functionally involved in substrate binding and catalysis, some of which distinguish the nematode enzymes from PEPCKs from other organisms. This analysis suggests a structural explanation for the kinetic differences seen between nematode and vertebrate PEPCKs and supports the hypothesis that nematode PEPCK is a target for selective inhibition. © 1993 Academic press, Inc.

引用

页码：155 / 161

页数：7

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