ENHANCEMENT OF HISTOLOGICAL DETAIL USING METANIL-YELLOW AS COUNTERSTAIN IN PERIODIC ACID SCHIFF HEMATOXYLIN STAINING OF GLYCOL METHACRYLATE TISSUE-SECTIONS

被引：142

作者：

QUINTEROHUNTER, I

GRIER, H

MUSCATO, M

机构：

[1] Florida Marine Research Institute, Department of Natural Resources, St Petersburg, FL, 33701

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1991年 / 66卷 / 04期

关键词：

STAINING; GLYCOL METHACRYLATE; CYTOLOGICAL DETAIL; PAS-HEMATOXYLIN; METANIL YELLOW;

D O I：

10.3109/10520299109109964

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Histological detail in sections from tissues embedded in glycol methacrylate was improved by counterstaining PAS/iron-hematoxylin stained sections with a dilute solution of metanil yellow. The addition of the counterstain increases contrast in tissue sections and highlights PAS-positive entities. The staining protocol provides sharp definition of tissue morphology, differentiates cell types and other tissue components and does not produce background staining.

引用

页码：169 / 172

页数：4