ISOLATION OF TYR-W-MIF-1 FROM BOVINE HYPOTHALAMI

被引：37

作者：

HACKLER, L

KASTIN, AJ

ERCHEGYI, J

ZADINA, JE

机构：

[1] VET ADM MED CTR,1601 PERDIDO ST,NEW ORLEANS,LA 70146

[2] TULANE UNIV,SCH MED,NEW ORLEANS,LA 70146

来源：

NEUROPEPTIDES | 1993年 / 24卷 / 03期

关键词：

D O I：

10.1016/0143-4179(93)90080-T

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Tyr-W-MIF-1 (Tyr-Pro-Trp-Gly-NH2) was recently isolated from human brain cortex. We have now isolated it from bovine hypothalami by solid phase extraction and several consecutive rpHPLC steps monitored by an RIA originally developed for the endogenous brain peptide Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2). Determination of the sequence of the purified material and comparison of its chromatographic behavior with synthetic Tyr-W-MIF-1 confirmed the structure. The synthetic peptide and the isolated material showed almost identical binding to mu opiate receptors.

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页码：159 / 164

页数：6

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