APPLICATIONS OF HETERODUPLEX ANALYSIS FOR MUTATION DETECTION IN DISEASE GENES

被引：37

作者：

GLAVAC, D

DEAN, M

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702

[2] NATL INST CHEM,LJUBLJANA,SLOVENIA

来源：

HUMAN MUTATION | 1995年 / 6卷 / 04期

关键词：

CYSTIC FIBROSIS; HLA TYPING;

D O I：

10.1002/humu.1380060402

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Double-stranded heteroduplex molecules that form between a mutant and wild-type DNA strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the HA method have been developed that increase the sensitivity of detection of single base pair alterations. (dagger)(C) 1995 Wiley-Liss, Inc.

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页码：281 / 287

页数：7

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