SINGLE-MOLECULE DETECTION - APPLICATIONS TO ULTRASENSITIVE BIOCHEMICAL-ANALYSIS

被引：28

作者：

CASTRO, A

SHERA, EB

机构：

[1] Biophysics Group, MS-D434, Los Alamos National Laboratory, Los Alamos, NM

来源：

APPLIED OPTICS | 1995年 / 34卷 / 18期

关键词：

SINGLE-MOLECULE DETECTION; SINGLE-MOLECULE ELECTROPHORESIS; ULTRASENSITIVE ANALYSIS; NUCLEIC-ACID DETECTION; LASER-INDUCED FLUORESCENCE;

D O I：

10.1364/AO.34.003218

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Recent developments in laser-based detection of fluorescent molecules have made possible the implementation of very sensitive techniques for biochemical analysis. We present and discuss our experiments on the applications of our recently developed technique of single-molecule detection to the analysis of molecules of biological interest. These newly developed methods are capable of detecting and identifying biomolecules at the single-molecule level of sensitivity. In one case, identification is based on measuring fluorescence brightness from single molecules. In another, molecules are classified by determining their electrophoretic velocities.

引用

页码：3218 / 3222

页数：5

共 16 条

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