ANTIGENIC ANALYSIS OF THE F-PROTEIN OF THE BOVINE RESPIRATORY SYNCYTIAL VIRUS - IDENTIFICATION OF 2 DISTINCT ANTIGENIC SITES INVOLVED IN FUSION INHIBITION

被引：26

作者：

MATHEISE, JP ^{[1
]}

WALRAVENS, K ^{[1
]}

COLLARD, A ^{[1
]}

COPPE, P ^{[1
]}

LETESSON, JJ ^{[1
]}

机构：

[1] CTR ECON RURALE,DIV IMMUNOL ANIM,MARLOIE,BELGIUM

来源：

ARCHIVES OF VIROLOGY | 1995年 / 140卷 / 06期

关键词：

D O I：

10.1007/BF01315410

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

From two independent fusions, fifteen MAbs directed to the F protein of the bovine respiratory syncytial virus (BRSV) were characterized by radioimmunoprecipitation assays. Competition binding assays among these MAbs identified two distinct antigenic sites (A and B) and one overlapping site (AB). Ah of the MAbs specific to epitopes belonging to site A neutralized the infectivity of the virus in vitro and recognized human and bovine RSV strains. Only two out of the five MAbs directed to epitopes of site B were neutralizing and three reacted with ah of the RSV strains tested, suggesting that the epitopes constituting this domain present heterogeneous characteristics. In each of sites A and B, one of the neutralizing MAbs also inhibited cell fusion. The biological relevance of these domains was established by competing representative MAbs and sera from BRSV-infected calves.

引用

页码：993 / 1005

页数：13

共 31 条

[1] CHARACTERIZATION OF 2 ANTIGENIC SITES RECOGNIZED BY NEUTRALIZING MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE FUSION GLYCOPROTEIN OF HUMAN RESPIRATORY SYNCYTIAL VIRUS [J].