GLOMERULAR BINDING OF ANGIOTENSIN-II IN THE RAINBOW-TROUT, SALMO-GAIRDNERI

Isolated glomeruli of the rainbow trout have been exposed in vitro to125I-angiotensin II (0.88 × 10-9 M) and binding sites located by light-microscopic autoradiography. These studies provide evidence of specific binding of angiotensin II by glomeruli. Binding was significantly inhibited by excess (10-5 M) unlabelled angiotensin II, but a high degree of non-specific binding also occurred. The mammalian competitive antagonist, saralasin (3 × 10-7 M) did not influence125I-angiotensin II binding to fish glomeruli. Intense binding of125I-angiotensin II was noted at the vascular pole of some glomeruli. © 1990 Springer-Verlag.