CA2+ AND IN-VITRO KAINATE DAMAGE TO CORTICAL AND HIPPOCAMPAL SMI-32(+) NEURONS

被引:12
作者
BURKE, SJ [1 ]
YIN, HZ [1 ]
WEISS, JH [1 ]
机构
[1] UNIV CALIF IRVINE, DEPT NEUROL, IRVINE, CA 92717 USA
关键词
PYRAMIDAL NEURON; GLUTAMATE; AMPA; KAINATE; CELL CULTURE; CORTEX; HIPPOCAMPUS; NEUROTOXICITY; NEUROFILAMENT; CALCIUM;
D O I
10.1097/00001756-199503000-00011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
SMI-32 is a monoclonal antibody to non-phosphorylated neurofilament epitopes, which labels subsets of pyramidal neurons prone to degeneration in Alzheimer's disease. We found SMI-32 to identify a small minority of neurons in dissociated cultures of murine cortex and hippocampus (SMI-32(+) neurons). Labeled neurons, which were larger than average and were often immunoreactive for GABA, were preferentially destroyed by brief kainate exposures. This rapidly triggered kainate damage to SMI-32(+) neurons was dependent upon the presence of Ca2+ in the media during the toxic exposure. Furthermore, most SMI-32(+) neurons in both cortex and hippocampus were subject to kainate-activated cobalt uptake, a histochemical procedure that marks cells with Ca2+ permeable AMPA/kainate channels. The unusual vulnerability of cortical and hippocampal SMI-32(+) neurons to AMPA/kainate receptor-mediated injury may result from rapid Ca2+ entry through Ca2+ permeable AMPA/kainate receptor-gated channels.
引用
收藏
页码:629 / 632
页数:4
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