BILIRUBIN IS OXIDIZED IN RATS TREATED WITH ENDOTOXIN AND ACTS AS A PHYSIOLOGICAL ANTIOXIDANT SYNERGISTICALLY WITH ASCORBIC-ACID IN-VIVO

被引:69
作者
YAMAGUCHI, T [1 ]
HORIO, F [1 ]
HASHIZUME, T [1 ]
TANAKA, M [1 ]
IKEDA, S [1 ]
KAKINUMA, A [1 ]
NAKAJIMA, H [1 ]
机构
[1] NAGOYA UNIV, DEPT APPL BIOL SCI, NUTR BIOCHEM LAB, NAGOYA, AICHI 46401, JAPAN
关键词
D O I
10.1006/bbrc.1995.2250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined the possibility that bilirubin physiologically acts as an antioxidant by using scurvy-prone ODS-od/od rats treated with endotoxin (lipopolysaccharide: LPS). Recently, bilirubin oxidative metabolites were isolated from human urine and named biotripyrrin-a and biotripyrrin-b. The LPS injection markedly increased bilirubin oxidative metabolites in urine of rats fed an ascorbic acid-free diet. This increase was supressed by feeding an adequate amount of ascorbic acid, a physiological antioxidant. The concentrations of biotripyrrin-a and -b in urine collected 6.5-10 h after the LPS injection were lower in rats fed an ascorbic acid-supplemented diet than in rats fed an ascorbic acid-free diet. Moreover, feeding with ascorbic acid suppressed the elevation of hepatic mRNA level of heme oxygenase-1, the rate-limiting enzyme of bilirubin biosynthesis, in rats injected with LPS. These findings suggest that bilirubin is oxidized in rats treated with LPS and acts as a physiological antioxidant synergistically with ascorbic acid in vivo. (C) 1995 Academic Press. Inc.
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页码:11 / 19
页数:9
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