EXCISION AND TRANSPOSITION OF 2 DS TRANSPOSONS FROM THE BRONZE MUTABLE-4 DERIVATIVE-6856 ALLELE OF ZEA-MAYS L

The regulatory mutation bronze mutable 4 Derivative 6856 (bz-m4 D6856) contains a complex 6.7 kb Dissociation (Ds) element tagged with a duplication of low copy bz 3′ flanking sequences (Klein et al. 1988). This creates a unique opportunity to study the transposition of a single member of the repetitive family of Ds elements. Eighteen full purple revertants (Bz′ alleles) of bz-m4 were characterized enzymatically and by genomic mapping. For 17 of the Bz′ alleles, reversion to a wild-type phenotype was caused by excision of the 6.7 kb Ds transposon. Nine of these Bz′ alleles retained the transposon somewhere in their genome. In this study we show that like Ac (Schwartz 1989; Dooner and Belachew 1989), the 6.7 kb Ds element can transpose within a short physical distance, both proximal and distal to its original position. Additional bz sequences have been mapped immediately distal to the mutant locus in bz-m4 D6856; genetic evidence suggests these are flanked by two additional Ds elements. The remaining Bz′ revertant, Bz′ :107, arose from excision of a more complex 13 kb Ds element. © 1990 Springer-Verlag.