SUGAR ESTERIFICATION CATALYZED BY ALKYLATED TRYPSIN IN DIMETHYLFORMAMIDE

被引：11

作者：

AMPON, K

SALLEH, AB

TEOH, A

YUNUS, WMZW

RAZAK, CNA

BASRI, M

机构：

[1] Penyelidikan Teknologi Enzim and Mikrob, Fakulti Sains and Pengajian Alam Sekitar, Universiti Pertanian Malaysia, Serdang

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 01期

关键词：

D O I：

10.1007/BF01033511

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modification with the respective aldehydes. These alkylated trypsins were more stable in DMF compared to the native unmodified enzyme at temperatures between 26 and 60-degrees-C.

引用

页码：25 / 30

页数：6

共 9 条

[1]

AMPON K, 1991, IN PRESS ENZYME MICR

[2] MEASUREMENT OF AMINO GROUPS IN PROTEINS AND PEPTIDES [J].

FIELDS, R .

BIOCHEMICAL JOURNAL, 1971, 124 (03) :581-&

[3]

FRETHEIM K, 1979, INT J PEPT PROT RES, V14, P451

[4]

KLIBANOV A M, 1986, Chemtech, V16, P354

[5] ASYMMETRIC TRANSFORMATIONS CATALYZED BY ENZYMES IN ORGANIC-SOLVENTS [J].

KLIBANOV, AM .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (04) :114-120

[6]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[7] MICROENVIRONMENTAL EFFECTS ON ENZYME CATALYSIS - A KINETIC-STUDY OF HYDROPHOBIC DERIVATIVES OF CHYMOTRYPSIN [J].

REMY, MH ;

BOURDILLON, C ;

THOMAS, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 829 (01) :69-75

[8]

RIVA S, 1986, J AM CHEM SOC, V108, P5638

[9] REGIOSELECTIVE ACYLATION OF SECONDARY HYDROXYL-GROUPS IN SUGARS CATALYZED BY LIPASES IN ORGANIC-SOLVENTS [J].

THERISOD, M ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (13) :3977-3981

← 1 →