REFOLDING KINETICS OF PIG MUSCLE AND YEAST 3-PHOSPHOGLYCERATE KINASES AND OF THEIR PROTEOLYTIC FRAGMENTS

被引：24

作者：

SEMISOTNOV, GV ^{[1
]}

VAS, M ^{[1
]}

CHEMERIS, VV ^{[1
]}

KASHPAROVA, NJ ^{[1
]}

KOTOVA, NV ^{[1
]}

RAZGULYAEV, OI ^{[1
]}

SINEV, MA ^{[1
]}

机构：

[1] HUNGARIAN ACAD SCI,BIOL RES CTR,INST ENZYMOL,H-1361 BUDAPEST 5,HUNGARY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16474.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The time course of refolding of both pig muscle and yeast 3-phosphoglycerate kinase (molecular masses about 47 kDa), as well as their proteolytic C-terminal fragments (30 and 33 kDa, respectively) has been investigated. Very similar refolding kinetics (with half-time between 80-120 s, at 20-degrees-C) were observed by fluorescence and ultraviolet absorbance spectroscopy, as well as by activity measurements, for the intact enzyme from both sources. This time course appears not to depend on the time the protein spends in the unfolded state, i.e. it is certainly not controlled by proline isomerization. Furthermore, after removal of a large N-terminal part (molecular mass of about 18 kDa for pig muscle enzyme or 13 kDa for yeast enzyme) of the molecule by proteolysis, refolding of the remaining C-terminal fragment of both proteins follows kinetics virtually indistinguishable from those of the intact protein molecule.

引用

页码：1083 / 1089

页数：7

共 36 条

[1] THE FOLDING AND MUTUAL INTERACTION OF THE DOMAINS OF YEAST 3-PHOSPHOGLYCERATE KINASE [J].

ADAMS, B ;

BURGESS, RJ ;

PAIN, RH .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 152 (03) :715-720

[2] KINETIC-STUDIES OF THE UNFOLDING-REFOLDING OF HORSE MUSCLE PHOSPHOGLYCERATE KINASE INDUCED BY GUANIDINE-HYDROCHLORIDE [J].

BETTON, JM ;

DESMADRIL, M ;

MITRAKI, A ;

YON, JM .

BIOCHEMISTRY, 1985, 24 (17) :4570-4577

[3] DETECTION OF INTERMEDIATES IN THE UNFOLDING TRANSITION OF PHOSPHOGLYCERATE KINASE USING LIMITED PROTEOLYSIS [J].

BETTON, JM ;

DESMADRIL, M ;

YON, JM .

BIOCHEMISTRY, 1989, 28 (13) :5421-5428

[4] CONSIDERATION OF POSSIBILITY THAT SLOW STEP IN PROTEIN DENATURATION REACTIONS IS DUE TO CIS-TRANS ISOMERISM OF PROLINE RESIDUES [J].

BRANDTS, JF ;

HALVORSON, HR ;

BRENNAN, M .

BIOCHEMISTRY, 1975, 14 (22) :4953-4963

[5] REVERSIBLE UNFOLDING OF YEAST 3-PHOSPHOGLYCERATE KINASE [J].

BURGESS, RJ ;

PAIN, RH .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1977, 5 (03) :692-694

[6]

CREIGHTON TE, 1990, BIOCHEM J, V270, P1

[7] EFFECTS OF SUBSTRATES ON THE HEAT-STABILITY AND ON THE REACTIVITIES OF THIOL-GROUPS OF 3-PHOSPHOGLYCERATE KINASE [J].

CSERPAN, I ;

VAS, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 131 (01) :157-162

[8] NUCLEAR MAGNETIC-RESONANCE STUDIES OF ISOLATED STRUCTURAL DOMAINS OF YEAST PHOSPHOGLYCERATE KINASE [J].

FAIRBROTHER, WJ ;

MINARD, P ;

HALL, L ;

BETTON, JM ;

MISSIAKAS, D ;

YON, JM ;

WILLIAMS, RJP .

PROTEIN ENGINEERING, 1989, 3 (01) :5-11

[9] AN EARLY IMMUNOREACTIVE FOLDING INTERMEDIATE OF THE TRYPTOPHAN SYNTHASE-BETA-2 SUBUNIT IS A MOLTEN GLOBULE [J].

GOLDBERG, ME ;

SEMISOTNOV, GV ;

FRIGUET, B ;

KUWAJIMA, K ;

PTITSYN, OB ;

SUGAI, S .

FEBS LETTERS, 1990, 263 (01) :51-56

[10] UNFOLDING AND REFOLDING OF THE CONSTANT FRAGMENT OF THE IMMUNOGLOBULIN LIGHT CHAIN CONTAINING AN INTRAMOLECULAR MERCURY BRIDGE [J].

GOTO, Y ;

HAMAGUCHI, K .

JOURNAL OF BIOCHEMISTRY, 1986, 99 (05) :1501-1511

← 1 2 3 4 →